Vector insertion mutagenesis of Rhizobium sp. strain ORS571: direct cloning of mutagenized DNA sequences
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چکیده
منابع مشابه
Rhizobium sp. strain ORS571 grows synergistically on N2 and nicotinate as N sources.
Rhizobium sp. strain ORS571 conducts synergistic, free-living N2 fixation and nicotinate oxidation. Explicitly, ORS571 is able to fix N2 aerobically because 6-OH-nicotinate acts as an intracellular O2 sink. Because 6-OH-nicotinate oxidation is mandatory for aerobic, free-living N2 fixation and because the synergistic processes yield ammonium from substrates (as the nitrogen source for growth), ...
متن کاملNicotinic acid requirement and degradation by Sesbania rhizobium strain ORS571
Sevhtrriia rliizobiuni strain ORS57 1. which grows in the free-living state at the expense of N, or ammonia. requires nicotinic acid as a growth factor and also uses this vitamin as a nitrogen source in culture medium devoid of ammonia. Consequently, under nitrogen-fixing conditions, Sesbania rhizohiw1 requires about 10-times more nicotinic acid than when grown in the presence of ammonia. The p...
متن کاملTwo genes that regulate exopolysaccharide production in Rhizobium sp. strain NGR234: DNA sequences and resultant phenotypes.
Two closely linked genes involved in the regulation of exopolysaccharide (EPS) production in Rhizobium sp. strain NGR234, exoX and exoY, were sequenced, and their corresponding phenotypes were investigated. Inhibition of EPS synthesis occurred in wild-type strains when extra copies of exoX were introduced, but only when exoY had been deleted or mutated or was present at a lower copy number. Nor...
متن کاملThe fast-growing Rhizobium strain ORS571, isolated from stem nodules of the tropical legume
In Rhizobium ORS571, which is able to form both root and stem nodules on the tropical plant Sesbania rostrata and to grow in the free-living state at the expense of N,, two copies of the nijH gene, which codes for the nitrogenase Fe-protein, were characterized. One copy, nijHl, was localized in the nijHDK operon; the other, nzjH2, was localized elsewhere. nijH1 and nijH2 differed in only six nu...
متن کاملThree replicons of Rhizobium sp. Strain NGR234 harbor symbiotic gene sequences.
Rhizobium sp. strain NGR234 contains three replicons: the symbiotic plasmid or pNGR234a, a megaplasmid (pNGR234b), and the chromosome. Symbiotic gene sequences not present in pNGR234a were analyzed by hybridization. DNA sequences homologous to the genes fixLJKNOPQGHIS were found on the chromosome, while sequences homologous to nodPQ and exoBDFLK were found on pNGR234b.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.162.1.317-323.1985